Comparative Analysis of Cellulosic Ethanol Production from Lignocellulosic Substrate Moringa oleifera Using Kluyveromyces marxianus and Zymomonas mobilis

In the current investigation, woody stem of Moringa oleifera was processed by chipping and milling subsequently exposed to a combination pretreatments involving 3% v/v solution nitric acid autohydrolysis. The simultaneous saccharification fermentation (SSF) pretreated hydrolysate M. conducted using Zymomonas mobilis Kluyveromyces marxianus in occurrence commercial cellulase enzyme, Tween 80, sodium azide. process parameters for Z. were optimized individually, including substrate concentration 5% (w/v), inoculum (v/v), pH 5.4, temperature 34 °C. Similarly, K. marxianus, with an 5.1, 41 highest cellulosic ethanol achieved micro-organism after period 96 h.